Enliten atp assay promega madison wi
WebPromega ENLITEN™ ATP Assay System The ENLITEN ATP Assay System is used to measure ATP levels for the indirect detection of biocontamination. Supplier: Promega FF2000 Catalog No. PR-FF2000 $390.00 / Each of 1 Qty Check Availability Add to cart WebNov 5, 2024 · Supernatant was collected and analyzed with ENLITEN ATP assay (Promega, Madison, WI, USA) and HMGB1 ELISA kit II (Shino-Test, Kanagawa, Japan). Western blot analysis K7M2 and NHOS cells, seeded in a 100-mm dish at a density of 2 × 10 5 cells/dish, were infected with OBP-502 at MOIs of 0, 10, 50, 100, 500, and 1000 …
Enliten atp assay promega madison wi
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WebATP bioluminescence. ATP releasing agent (Somalyze), Tris-acetate buffer, ATP-monitoring reagent (firefly luciferase, D-luciferin) and ATP-standard (EG+G. Wallac, Turku, Finland) are needed for the assay. 100 µL of ATP releasing agent was added to each well of a 96 well microtitre tissue culture plate (Greiner, Germany), containing 100 µL of ... WebATP release was assessed with an ENLITEN ATP Assay System ( FF2000, Promega ). HMGB1 and HSP-90 release into the medium was measured with an HMGB1 ELISA kit …
WebMay 9, 2024 · After being treated for different time points (0.5, 1, 1.5, and 2 h), the supernatant of cells was analyzed using the ENLITEN ATP luciferase assay kit (Promega, Madison, WI, USA). The level of extracellular ATP chemiluminescence was measured using the Wallac VICTOR 3 Multilabel plate reader (PerkinElmer, Shelton, CT, USA). Webendogenous ATP and the accessibility of exogenous ATP to the luminal side of the endoplasmic reticulum (ER) where the active site of UGT is located. Gefitinib, This article …
WebENLITEN® ATP Assay System 100 assays ₩ 377,000 Your price: Log in The ENLITEN® ATP Assay System can be used to measure ATP levels for the indirect detection of biocontamination on food processing surfaces, in cosmetics and beverages or to assay for enzymes that degrade ATP and to quantitate ATP in biological fluids. WebEnter the email address you signed up with and we'll email you a reset link.
WebAug 30, 2024 · We used an ATP assay kit (Enliten, Promega, Madison, WI, USA) to estimate the ATP concentration in the supernatant by measuring in the luminescence channel of a Cytation 5 plate reader (BioTek Instruments, Inc., Winooski, VT, USA). The results are expressed in nanomolar ATP per milligram of proteins. 首 おでこWebThe Enliten ATP Assay System is a bioluminescence detection kit which is designed to measure down to 0.1 femtomoles of ATP. As shown in Figure 5, TopCount achieved this detec- tion limit, 0.1 femtomoles, and had a linear range of 10-12to 10-15 moles. tarikh bayaran dividen kwsp 2022Web2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB306 · Revised 5/09 www.promega.com 1. Description The CytoTox-ONE™ Homogeneous Membrane Integrity Assay(a) is a homogeneous, fluorometric method for estimating the number of … 首 おでき 原因