Webb9 apr. 2024 · 目的:发现与A相互作用的motif 方法:以构建到pGADT7载体上的A基因为诱饵,筛选酵母单杂交motif文库,经过对阳性克隆的基因检测、DNA测序和比对分析,确定与pGADT7-A相互作用的motif。 材料: 1.诱饵克隆:pGADT7-A 2.诱饵载体:pGADT7 3.文库酵母质粒:motif 4.Clontech酵母单杂交系统 5.培养基 Webb1)准备质粒:构建pHis2-bait质粒→提取质粒; 2)制备Y187感受态细胞:YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞;或者直接购买Y187感受态细胞(CC306); …
新-TF-centered Y1H筛选与转录因子A相互作用的motif实验结题报 …
http://www.labbase.net/Supply/SupplyItems-4229347.html WebbOur Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbA r) as a stringent, highly selective, and easy-to-use reporter. ctrl + a no word
pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …
Webbp53-His,pLacZ,pHis,pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主,特殊要求的菌种可用其它方法进行保存,保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主,在0℃以下保存,保存期为3-6个月。. 3、应用菌株 … WebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by … earth trn701