Phis2

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WebbVector Map pGADT7-Rec Vector (Not sold separately) Sold as a part of Cat. Nos. 630490 & 630491 (102016) Page 2 of 4 . Figure 2. pGADT7-Rec Vector cloning site. WebbShare your videos with friends, family, and the world

Transcription Factor-Centered Yeast One-Hybrid Assay

WebbOur dedication to customer support brings unique and effective solutions to your specific challenges and applications. Partnering with Takara Bio allows you to: Collaborate with … WebbpHIS2 Vector Information Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3705-5 2 Version No. PR32049 Notice to Purchaser This product is intended to be … smallest monitor in the world

SnapGene Software for everyday molecular biology

Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … smallest monitor lcd

petunia transcription factor, PhOBF1, regulates flower senescence …

CmMYB9a activates floral coloration by positively regulating

Webb10 aug. 2015 · The yeast one-hybrid assay was carried out using the Matchmaker one-hybrid system following the manual (Clontech, Palo Alto, CA, USA). The promoter … http://www.honorgene.com/product_list/carrier_library/123112.html smallest monitor lizard species for petsWebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … smallest monitor species

"Webbxho1和sac1的酶切位点是什么？它识别的特定基因是体内3广a泛存在的siRNA和miRNA前体。Dicer酶的PAZ结构域特异性地识别 0’端有 5个j碱基突出的 dsRNA,之w后由RNase III结构域剪切8生成siRNA或microRNA。 2011-10-27 8:58:59 " - Phis2

Phis2

p53-His2 plasmid,p53-His2,p53-His2 plasmid,p53-His2 …

Webb12 mars 2024 · The pHIS2-MdANSPro and the pHIS2-MdUFGTPro vectors were respectively transformed into Y187 yeast strains that were cultivated in -T-H selective media containing different concentrations of 3-AT. Reduced yeast growth was observed with increasing 3-AT concentration, and yeast could not grow on -T-H selective media … WebbStack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, …

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WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector harboring the GAL4 activation domain, which was regarded as the pray construct. The recombinant plasmids were co-transformed into Saccharomyces cerevisiae strain Y187.

Webb29 okt. 2024 · To test whether CmMYB9a directly binds to the promoters of these four structural genes, yeast one-hybrid assays were performed with co-transformation of pGADT7 (AD)-CmMYB9a constructs and pHIS2 vectors containing the promoters. Co-transformation of the AD vector and these pHIS2 constructs served as negative controls. Webb原创不易，转载请注明来源于Coolaber公众号！本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例， …

Webb8 juni 2014 · 本研究根据植物胚柄特异表达基G564启动子关键序列(GAAAAGCGAA)合成次串联重复序列，并与报告质粒pHIS2．1连接构建诱饵载体pHIS2．1．G564．5A；以纯化的拟南芥rabidopsisthaliana)幼嫩种子mRNA模板，合成SMARTTM和CDS1I锚定末端dscDNA：并将dscDNA与线性化融合表达载体pGADT7一Rec2 ... Webbphis-docs-community Overview. This repository contains OpenSILEX PHIS users documentation. Background. PHIS (Phenotyping Hybrid Information System) is …

WebbpHIS-Hsv2 was a gift from James Hurley (Addgene plasmid # 42524 ; http://n2t.net/addgene:42524 ; RRID:Addgene_42524) For your References section: Two …

Webb酵母单杂. 使用pHIS2载体，将启动子序列构建在HIS3报告基因前面的多克隆位点上，对应的转录因子构建在AD载体上即可。. 使用Y187菌株，它相对于AH109菌株在基因组上少 … smallest monitor lizard speciesWebbCo-transformation of yeast strain Y187 with pGADT7 and pHIS2 . Point-to-point verification. 1) Culture on the corresponding verification plate. 2) Drip X-α-Gal working solution on the … song marvin gaye what\u0027s going onWebb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors … smallest monkey in the boholWebbY187-pHis2系统酵母单杂原理：当猎物蛋白（Prey）结合到诱饵序列（Bait DNA）上，GAL4 AD就会激活下游报告基因HIS3的表达，能够在含有3-AT的培养基上生长，从而确定DNA和蛋白有相互。. 本方案中检测诱饵酵母菌株的3-AT最佳使用浓度和互作验证都采用了稀释点板的方法 ... song mary did you know by the braxtonsWebb载体介绍. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use … smallest monitor sizeWebbPHIS2 Login for pricing STARTER FLUOR 4-22W 220-240V 2P SER See Related Products. Standard Features; Additional Features; Part No. PHIS2. Description. STARTER FLUOR 4 … smallest monokey top boxWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … smallest monitor speakers