WebOct 12, 2012 · The p38 kinase phosphorylated the T249A mutant as efficiently as WT Atg5, whereas the T75A mutant was not phosphorylated by p38, indicating that p38 phosphorylates Atg5 at T75 . Figure 5 Atg5 is a ... WebFeb 12, 2024 · During the course of studies using [γ-32 P]ATP labeling of recombinant proteins in vitro, we observed that when ZAP-70 and p38 were coincubated, there was, in addition to the expected labeled band at 38 …
p38 MAPK Antibody Cell Signaling Technology
WebApr 20, 2024 · Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.1% Triton X-100, and incubated with Alexa Fluor 488 phalloidin (1:200) for 45 min, followed by 4′,6-diamidino-2-phenylindole (DAPI) staining. The cell size was measured using NIS Elements Software (Nikon, Japan). Cell Viability WebJul 23, 2004 · Samples were analyzed using 4–12% SDS-PAGE followed by immunoblotting using an antibody specific for the phosphorylated form of p38. Bands were normalized to total p38. *, ... Download : Download full-size image; F ig. 6. A p38 inhibitor blocks glucose-mediated activation of the human IL-8 promoter. arabic name hadi meaning
MafA transcription factor is phosphorylated by p38 MAP kinase
WebNov 27, 2009 · The primary antibodies used were: (1) anti-p53 antibody directed at residues 50–100 of human tumour protein p53 (Bethyl Laboratories, Montgomery, USA), dilution 1:2000, (2) anti-p38 MAP kinase antibody derived from human p38 MAP kinase sequence (Cell Signaling Technology, Beverly, MA, USA), dilution 1:2000, (3) anti-phosphorylated … WebJul 4, 2005 · In contrast, only the phosphorylated forms could be detected in band B, suggesting that Ser272 was fully phosphorylated when p38 was activated (Fig. 4 C). The … WebPhosphorylated (p)-P38, p-CREB, and MMP13 were evaluated by Western blot analysis. Anisomycin was used to activate P38, and p-P38, p-CREB, and MMP13 were evaluated by immunofluorescence and Western blot analysis.Results: Safranin O staining showed that the extracellular matrix degraded in humans with OA and ANS-induced mouse cartilage … baixar nha rei yasmine